The activity of urease is hard to recover entirely with regeneration solution after contacting inhibitors for many times . for this reason , a renewable urease biosensor is highly desired . in chapter 3 , a renewable potentiometric urease inhibition biosensor based on self - assembled gold nanoparticles has been developed for the determination of mercury ions 2 .重金屬離子對脲酶的抑制作用是不可逆的,因此基于對脲酶抑制來檢測汞離子的傳感器在多次與抑制劑接觸后,很難使用再生溶液將脲酶的活性完全恢復(fù),有必要研究一種可更新的脲酶傳感器。